Mapping a male-fertility restoration locus for the A4 cytoplasmic-genic male-sterility system in pearl millet using a genotyping-by-sequencing-based linkage map

Table 2 Quantitative trait loci for pollen production and selfed seed set (traits indicating male-fertility restoration of the A₄ CMS system) and plant height

	LG^b	Position (cM)	Support Interval (cM)	LOD	Left SNP^c	Right SNP^c	R²_adj	Genetic Effect		Cross-Validation Frequency (%)
	LG^b	Position (cM)	Support Interval (cM)	LOD	Left SNP^c	Right SNP^c	R²_adj	Additive	Dominant	Cross-Validation Frequency (%)
Pollen Production^a	2	44	42–45	7.86	S2_110825781	S2_195649011	14.48	0.381	0.437	97
Selfed Seed Set	2	44	42–45	5.39	S2_110825781	S2_195649011	9.98	0.349	0.382	38
Plant Height	4	38	37–40	11.52	S4_48328719	S4_70429741	24.54	26.686	27.697	69

^aPollen Production was scored as 0 = no pollen production, 1 = pollen production; Selfed Seed Set was scored as 1 = up to 5% seed set, 2 = 5 to 50% seed set, 3 = more than 50% seed set when plants were self-pollinated; Plant Height was measured in cm
^bLG, Linkage group; 1-LOD Support Interval; LOD, logarithm of odds; R²_adj, adjusted percentage of observed phenotypic variance; Frequency, QTL frequency determined by cross-validation
^cThe genomic context of all SNPs in the linkage map, along with their physical location in the current pearl millet reference genome (NCBI Assembly [27]), are presented in Additional file 2

ISSN: 1471-2229