Fig. 4
From: Chilling-induced DNA Demethylation is associated with the cold tolerance of Hevea brasiliensis
Effect of long-term cold treatment on phenotype and gene expression patterns. Seedlings of H. brasiliensis were treated at 19 °C for 1 month (1-CT), transferred to 28 °C for recovery for 1 month, then treated at 19 °C for 1 month (2-CT). After recovery for 1 month, the seedlings were again cold-treated for 1 month (3-CT). After each treatment, the phenotypes were photographed (a) and leaf samples were collected. The expression levels of cold-related genes (b) and methylation-related genes (c) were analysed by qRT-PCR. Values are presented as the mean standard error of three independent biological replicates. Different letters indicate significant differences (P < 0.05) according to one-way analysis of variance