Fig. 10

Validation of Slcirc108 parent gene Solyc07g043420.2.1 with virus-induced gene silencing. a Cotyledon agroinfiltration of TRV vectors with TYLCV infection was performed in the susceptible tomato at the cotyledon stage. Plants treated with the pTRV empty vectors showed the susceptible phenotype (Left). Susceptible plantlets treated with the pTRV1 and pTRV2-Solyc07g043420.2.1 had no symptoms (right). b Relative expression levels of Solyc07g043420.2.1 using real-time RT-PCR analysis in the VIGS-treated tomato plants 20 days after agroinfiltration with TRV2 vectors. c Relative expression levels of Slcirc108 using real-time RT-PCR analysis in the VIGS-treated tomato plants 20 days after agroinfiltration with TRV2 vectors. d TYLCV accumulation in the Solyc07g043420.2.1 silenced plants was estimated from the total genomic DNA by quantitative RT-PCR