Fig. 5

Ectopic expression of OsWRKY42 suppresses salt and cellulase induced expression of JA biosynthesis and responsive genes in Arabidopsis transgenic lines. Expression of JA biosynthesis and response genes was measured in 35S::OsWRKY42 transgenic lines and wild type (Col-0) Arabidopsis plants after salt and cellulase treatment. a. For salt treatment, one-week old seedlings (wild type or 35S::OsWRKY42) were treated for 12 h with either MS or MS with 150 mM NaCl. b. For cellulase treatment, leaves of three weeks old Arabidopsis plants (wild type or 35S::OsWRKY42) were infiltrated with 2 U/ml of cellulase (Sigma). Leaves were harvested after 12 h and processed for qPCR analysis. The graph represents the relative fold change using expression values from wild type or 35S::OsWRKY42 lines treated with water. c. Leaves of fourteen days old rice seedlings were syringe infiltrated with either LBA4404 or LBA4404/pH7FWG2::OsWRKY42. Twelve hours post treatment, leaves were harvested and processed for qRT-PCR. The relative fold change was calculated over control (leaves infiltrated only with LBA4404). AtUBQ5 and OsGAPDH were used as endogenous controls for rice and Arabidopsis, respectively. The average from three biological replicates is plotted on the graph. The error bar represents standard deviation. Data was analysed using the Student’s t-test for independent means. The asterisk above the bar indicates significant difference with p value < 0.05. The Arabidopsis experiments were repeated in three independent 35S::OsWRKY42 transgenic lines