Fig. 4

Analysis of GsMATE protein localization. a Localization of GsMATE-GFP fusion protein or GFP alone in Arabidopsis protoplasts; b and e Corresponding bright-field images; c and f. Merged images; d pYL322-d1-eGFP vector. The GsMATE-GFP fusion vector was constructed by cloning the full coding sequence of GsMATE (without TAA) into the BamHI and KpnI sites of pYL322-d1-eGFP vector. Arabidopsis protoplasts were transformed using the heat-shock method. After 24 h, GFP or GsMATE-GFP fusion protein expression (driven by the CaMV 35S promoter) was visualized by fluorescence microscopy