Fig. 3

Pollen tube growth in pistils in ANN5 RNAi-silenced lines. Pollen tubes were fixed and stained with Aniline Blue 6 h after hand-pollination of (a) wild-type Col-0, (b) ANN5-RNAi_15, (c) ANN5-RNAi_13, and (d) ANN5-OE_2 plants. Aniline blue staining of pollen tubes was performed as described by [19]. Yellow arrows indicate pollen tube length measured from the top of style to the front of the longest pollen tube. (e) Average lengths of pollen tubes in pistils. n = 3 independent experiments. Asterisk indicates significant difference compared with the wild type (one-way ANOVA, Dunnett post hoc test, (^#p = 0.0501; *p < 0.05; **p < 0.01; ***p < 0.001). See also Additional file 1: Figure S5. Scale bars = 200 μm