Fig. 9

STARP-based expression analysis of the Rec8 homoeoalleles (TtRec8-A1, TtRec8-B1, TaRec8-D1, TuRec8-A1, and AetRec8-D1) in polyploid wheat. a Primer specificity tests (32 PCR cycles) in the genomic DNA of LDN, LDN 1D(1A), LDN 1D(1B), T. urartu (wheat A genome donor), and Ae. tauschii (wheat D genome donor). b PCR amplification efficiency tests of the allele-specific primers in the genomic DNA of LDN, LDN 1D(1A), and LDN 1D(1B) at different PCR cycles (26, 30, 34, and 38). c PCR amplification of the cDNA from the anthers at the meiotic interphase (I) and pachytene (P) stages in LDN, LDN 1D(1A), and LDN 1D(1B) with different primer sets at different PCR cycles (25, 30; 26, 31; and 30, 35). d PCR amplification of the genomic DNA and cDNA from the anthers at I and P stages of LDN × Ae. tauschii, LDN 1D(1A) × Ae. tauschii, and LDN 1D(1B) × Ae. tauschii hybrids with different primer sets at different PCR cycles (25, 30; 26, 31; and 30, 35)