Towards reducing the immunogenic potential of wheat flour: omega gliadins encoded by the D genome of hexaploid wheat may also harbor epitopes for the serious food allergy WDEIA

Table 1 Identification of 2-DE spots from parental and mutant lines by MS/MS

Cultivar	Spot #	Predominant Protein	Accession Number	# Exclusive Unique Peptides	# Spectra	% Coverage
Keumkang	1	omega-5 gliadin	CAR82267	16	47	29
Keumkang	2	omega-5 gliadin	BAE20328	16	48	25
Keumkang	3	omega-5 gliadin	BAE20328	28	59	38
Keumkang	4	omega-5 gliadin	BAE20328	6	12	19
Keumkang	5	omega-5 gliadin	BAE20328	15	41	40
Keumkang	6	LMW-GS (i-type)^a	AAS10189	59	180	81
Keumkang	7	LMW-GS (s-type)^b	ACA63869	67	191	73
Keumkang	8	LMW-GS (s-type)^b	ACA63869	64	211	72
Keumkang	9	LMW-GS (s-type)^b	ACA63869	90	305	76
Keumkang	10	LMW-GS (s-type)^b	ACA63869	130	443	88
Keumkang	11	LMW-GS (s-type)^b	ACA63865	90	313	78
Keumkang	12	triticin	ACB41345	30	74	29
Keumkang	13	gamma gliadin	Bu-5^d	53	177	60
Keumkang	14	gamma gliadin	Bu-5^d	47	164	61
Keumkang	15	gamma gliadin	AGJ50340	46	164	62
Keumkang	16	LMW-GS (m-type)^c	ABC84361	66	179	74
Keumkang	17	LMW-GS (m-type)^c	ACA63871	70	357	84
Olgeuru	1	omega-5	AIU64835	7	26	48
Olgeuru	2	omega-5	AII26682	6	15	17
Olgeuru	3	omega-5	AII26682	4	15	16
Olgeuru	4	omega-5	CAR82266	6	13	25
Olgeuru	5	omega-5	BAE20328	36	75	45
Olgeuru	6	omega-5	AII26682	9	25	34
Olgeuru	7	LMW-GS (i-type)^a	BAB78764	60	175	77
Olgeuru	8	LMW-GS (s-type)^b	ACA63864	40	110	54
Olgeuru	9	LMW-GS (s-type)^b	ACA63868	44	124	62
Olgeuru	10	LMW-GS (s-type)^b	ACA63864	92	310	73
Olgeuru	11	LMW-GS (i-type)^c	ACY08811	47	111	69
Olgeuru	12	triticin	ACB41345	24	60	27
Olgeuru	13	gamma gliadin	AGO17716	23	51	45
Olgeuru	14	gamma gliadin	AGO17716	31	74	57
Olgeuru	15	LMW-GS (m-type)^c	ABC84361	60	164	74
Olgeuru	16	LMW-GS (m-type)^c	ACP27643	23	118	51
DH20	1	LMW-GS (i-type)^a	AAS10189	104	310	83
DH20	2	LMW-GS (i-type)^a	AAS10189	30	98	70
DH20	3	triticin	ACB41345	39	129	37
DH20	4	gamma gliadin	AGJ50340	49	161	58
DH20	5	LMW-GS (m-type)^c	ABC84361	60	164	74

ISSN: 1471-2229