Fig. 2
GLB1 transcription depends on the balance of ammonium and nitrate in the medium. a GLB1 transcript levels were determined in Chlamydomonas cells of cw15–325 strain grown in ammonium-containing medium and transferred to media containing 4 mM NO3−, 4 mM NO3− + 7.5 mM NH4+ or 4 mM NO3− + 1 mM NH4+. Values are means ± SE of three biological replicates and three technical replicates and are given as expression level relative to a house-keeping gene RACK1 that has a value of 1. Expression level at 0.5 h is considered as a control. The increase in NIT1 transcript abundance was used as a positive control (insertion). b GLB1 transcript levels were determined in Chlamydomonas cells of cw15–325 strain grown in ammonium-containing medium and transferred to media containing the indicated concentrations of NO3 − and 1 mM NH4+ for 2 h. Values are means ± SE of three biological replicates and three technical replicates and are given as expression level relative to a house-keeping gene RACK1 that has a value of 1