Fig. 2From: Genome-wide identification, molecular evolution, and expression analysis of auxin response factor (ARF) gene family in Brachypodium distachyon LSubcellular localization of five BdARF proteins in Arabidopsis thaliana protoplasts. Five proteins included BdARF1, BdARF4, BdARF5, BdARF17 and BdARF19. The localization of the nuclei was detected by 4′,6-diamidino-2-phenylindole (DAPI) staining. GFP: GFP fluorescence signal. Green fluorescence indicates the location of BdARFs in the Arabidopsis protoplasts; Chlorophyll: chlorophyll autofluorescence signal. Red fluorescent signal indicates the location of chloroplasts in protoplasts; DAPI: Blue fluorescence signal. Blue fluorescence indicates the location of the nucleus stained by DAPI; bright light: field of bright light; Merged: emergence of the GFP fluorescence signal, chlorophyll autofluorescence signal and bright light field; Nagtive: Wild-type (Clo) Arabidopsis protoplast cell. Scale bar = 5 μmBack to article page