Fig. 6

Cell wall thickening in interfascicular fibers and vessels in HyPRP1-silenced cotton plants at 14 days post inoculation with V. dahliae. a Cross-sections of the vascular bundle region of the control and HyPRP1-silenced plants. Scale bar = 10 μm. c, cortex; if, interfascicular fiber; v, vessel. a′, Measurement and statistical analysis of cell wall thickness in interfascicular fibers. b, Transmission electron micrographs of vessel walls of the control and HyPRP1-silenced plants. Scale bar = 2 μm. b′, Measurement and statistical analysis of cell wall thickness in vessels. 10-day-old seedlings with two fully expanded cotyledons were used for VIGS. After two weeks later HyPRP1-silenced plants were inoculated with V. dahliae. For each treatment, six separate plants were examined. 10 or more cells were measured for each plant. Data shown are means ± SE of three independent experiments. Asterisks indicate a statistically significant difference according to the non-parametric Mann Whitney test (**P < 0.01)