Fig. 1

Hypersensitive response, ROS burst and callose deposition induced by ptHR genes in N. benthamiana leaves. N. benthamiana leaves were infiltrated with buffer, EHA105 Agrobacterium strain, empty vector, pTRV₂-Avr4 and pTRV₂-Cf4 as positive control, and ptHR genes. Pictures were taken at 48 hpi. a-h HR induced by ptHR genes in N. benthamiana leaves treated with a buffer, b Agrobacterium EHA105 strain, c pTRV empty vector, d pTRV₁ as a control, e pTRV₂-Avr4 and pTRV₂-Cf4 as a positive control, f ptHR941, g ptHR375, and h ptHR293. i-l ROS accumulation induced by ptHR genes in N. benthamiana leaves treated with, i ptHR941 cloned in pTRV Ex vector, j pTRV empty vector, k ptHR941 cloned in pCAMBIA3301 vector, l pCAMBIA3301 empty vector. m-p Callose deposition induced by ptHR genes in N. benthamiana leaves treated with, m ptHR941 cloned in pTRV Ex vector, n pTRV empty vector, o ptHR941 cloned in pCAMBIA3301 vector, p pCAMBIA3301 empty vector. N benthamiana leaves were infiltrated with ptHR genes and empty vector (pTRV and pCAMBIA3301). Each experiment was repeated three times, and each time the same results were observed