Fig. 2

Effects of OsNCA1a and OsNCA1b on the activity and solubility of CATC as expressed in E. coli. (A) Effects of OsNCA1a and OsNCA1b on CAT activity. After proteins were inductively expressed, they were purified by passing a Ni-affinity column and then CAT activities were assayed; NCA1a, NCA1b, CATC: each was expressed alone; NCA1a + CATC or NCA1b + CATC: both were co-expressed; Data represent means ± SD of at least three replicate experiments. Different letters on the top of columns indicate significant difference at p < 0.05 according to Duncan’s multiple range test. (B) Proteins from supernatants, precipitates and whole E. coli cells were separately sampled and subjected to SDS-PAGE. I: insoluble (precipitate); S: soluble (supernatant); T: total proteins in whole cells. Molecular weight ladders (M) were loaded on the right lane