Table 1 Prediction of VvmiR160 target genes by bioinformatics
MiR-Acc. | MiRNA mature sequence | MiRNA length | Target_Acc. | Expectation | UPE | Target regions | Inhibition | Multiplicity |
|---|---|---|---|---|---|---|---|---|
VvmiR160a/b | TGCCTGGCTCCCTGAATGCCATC | 23 | VIT_218s0001g04180.1 (VvARF17) | 1 | 20.766 | CDS:1755–1777 | Cleavage | 1 |
23 | VIT_208s0040g01810.1 (VvARF10) | 1 | 23.188 | CDS:2389–2411 | Cleavage | 1 | ||
23 | VIT_208s0040g01810.3 (VvARF10) | 1 | 23.188 | CDS:2513–2535 | Cleavage | 1 | ||
23 | VIT_208s0040g01810.2 (VvARF10) | 1 | 23.188 | CDS:2389–2411 | Cleavage | 1 | ||
23 | VIT_208s0040g01810.4 (VvARF10) | 1 | 23.188 | CDS:2534–2556 | Cleavage | 1 | ||
23 | VIT_218s0001g04180.2 (VvARF17) | 1 | 20.766 | CDS:1755–1777 | Cleavage | 1 | ||
23 | VIT_213s0019g04380.1 (VvARF16) | 1 | 22.721 | CDS:1566–1585 | Cleavage | 1 | ||
23 | VIT_213s0019g04380.3 (VvARF16) | 1 | 22.721 | CDS:1566–1585 | Cleavage | 1 | ||
23 | VIT_213s0019g04380.4 (VvARF16) | 1 | 22.721 | CDS:1701–1720 | Cleavage | 1 | ||
23 | VIT_213s0019g04380.2 (VvARF16) | 1 | 22.721 | CDS:2147–2166 | Cleavage | 1 | ||
VvmiR160c/d/e | TGCCTGGCTCCCTGTATGCCA | 21 | VIT_218s0001g04180.1 (VvARF17) | 0.5 | 20.766 | CDS:1757–1777 | Cleavage | 1 |
21 | VIT_208s0040g01810.1 (VvARF10) | 0.5 | 23.188 | CDS:2391–2411 | Cleavage | 1 | ||
21 | VIT_208s0040g01810.3 (VvARF10) | 0.5 | 23.188 | CDS:2515–2535 | Cleavage | 1 | ||
21 | VIT_208s0040g01810.2 (VvARF10) | 0.5 | 23.188 | CDS:2391–2411 | Cleavage | 1 | ||
21 | VIT_208s0040g01810.4 (VvARF10) | 0.5 | 23.188 | CDS:2536–2556 | Cleavage | 1 | ||
21 | VIT_218s0001g04180.2 (VvARF17) | 0.5 | 20.766 | CDS:1757–1777 | Cleavage | 1 | ||
21 | VIT_213s0019g04380.1 (VvARF16) | 0 | 22.721 | CDS:1566–1585 | Cleavage | 1 | ||
21 | VIT_213s0019g04380.3 (VvARF16) | 0 | 22.721 | CDS:1566–1585 | Cleavage | 1 | ||
21 | VIT_213s0019g04380.4 (VvARF16) | 0 | 22.721 | CDS:1701–1720 | Cleavage | 1 | ||
21 | VIT_213s0019g04380.2 (VvARF16) | 0 | 22.721 | CDS:2147–2166 | Cleavage | 1 |