Table 1 Transformation efficiencies for rapid-cycling Brassica rapa
Method | Age of Seedlings (d) | Number of Seeds | Number of GFP-tagged T1 Seedlings | Transformation rate |
|---|---|---|---|---|
Vacuum infiltration | 22 | 1831 | 8 | 0.44% |
763 | 0 | 0.00% | ||
540 | 0 | 0.00% | ||
759 | 0 | 0.00% | ||
507 | 0 | 0.00% | ||
768 | 1 | 0.13% | ||
Vacuum infiltration | 14 | 2065 | 2 | 0.10% |
2669 | 4 | 0.15% | ||
2928 | 6 | 0.20% | ||
Floral dip | 14 | 1437 | 0 | 0.00% |
1295 | 2 | 0.15% | ||
775 | 0 | 0.00% | ||
745 | 1 | 0.13% | ||
1653 | 0 | 0.00% | ||
1307 | 1 | 0.08% | ||
1019 | 1 | 0.10% | ||
1585 | 0 | 0.00% | ||
1348 | 2 | 0.15% | ||
1640 | 0 | 0.00% | ||
Floral dip | 8 | 1621 | 1 | 0.06% |
1722 | 1 | 0.06% | ||
1712 | 3 | 0.18% | ||
529 | 0 | 0.00% | ||
934 | 0 | 0.00% | ||
710 | 0 | 0.00% | ||
Overall | 32,862 | 33 | 0.10% |