Fig. 4
Patchoulol content and genes expression pattern in patchouli leaves induced by MeJA. a GC-MS chromatogram of leaves extracts from plant sprayed with or without MeJA. b Contents of patchoulol in leaves significantly increased after MeJA treatment. The amount of patchoulol was first calculated comparing with external standard and then dividing by the fresh weight of the samples. Error bars indicate the SD of three biological replicates. ** indicates significant difference of the means at p < 0.01 between non-treantment (CK) and MeJA-treatment leaves for each parameter mesureed (n = 3). c Abundance of DEGs in leaf tissues in response to MeJA treatment. The heat map showing genes having a fold change ratio ≥ 2, with corrected p-value<0.01. d Abundance changes of transcripts involved in the cytosolic MVA, plastidial MEP, and P. cablin patchoulol biosynthesis pathways in response to MeJA. The expressions are indicated by heat map, estimated using Log2(foldchange) value for each transcript. Magenta means high expression and green means low expression, the color gradually from green to magenta represents gene expression abundance from low to high