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Fig. 3 | BMC Plant Biology

Fig. 3

From: Putative trehalose biosynthesis proteins function as differential floridoside-6-phosphate synthases to participate in the abiotic stress response in the red alga Pyropia haitanensis

Fig. 3

Analysis of the reaction products of the in vitro translation of the TPS domain from PhTPS1–4 of P. haitanensis using the E. coli BL21 cells expression system by SDS-PAGE. a Coomassie-stained gel. The arrows indicate proteins of the expected size for PhTPS1–4 (lane M, Maker; lane 1–4, PhTPS1–4, lane 5, negative control, with empty pET-28a or pET-28a-sumo vector). b Translated proteins were analyzed by western blot using a His-tag-specific antibody

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