Fig. 9
From: Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus
Twi1 in vitro glycosyltransferase activity assay using flavonoids as substrates. The Twi1 purified recombinant protein was tested for glycosyltransferase activity in vitro using UDP-glucose and the following flavonoids as sugar acceptors: apigenin, naringenin, quercetin and kaempferol. Samples were analysed by UPLC-Q-ToF-MS to detect sugar acceptor and glycoconjugate formation. Plus (+): glycoconjugate detected; Minus (−): glycoconjugate not detected