Fig. 1From: WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheatAssessment of off-target effects of gRNAs with a tiered k (mismatches) approach. CFD densities are plotted by k (mismatches) using a subset of approximately one million canonical (a) and non-canonical (b) on-target::off-target gRNA pairingsBack to article page