Fig. 3

Cloning and phylogeny analysis of LOW. a Association analysis based on Mutation Mapping Analysis Pipeline for Pooled RNA-seq method. The red region on chromosome 7 indicates strongest association and the red arrow marks the chromosome. b Physical map of the large region in Chromosome 7 of Vigna radiata, where LOW is located. Marker information (M) and recombination frequency (Rec.) are shown. In the lower lane, relative expression heat map of candidate genes between M9 and M10 is shown; the red arrow marks the Vr07g10060/LOC10676703. c The gene structure of LOW, nucleotide numbers, start and terminal codons are shown; the red arrow indicates the mutation. d Maximum-likelihood tree of LOW-like Dof genes from Aquilegia coerulea (Ac), Arabidopsis thaliana (At), Brassica rapa (Br), Capsella rubella (Cr), Glycine max (Gm), Medicago truncutula (Mt), Populus trichocarpa (Pt), Phaseolus vulgaris (Pv), Solanum lycopersicum (Sl), Solanum tuberosum (St) and Vigna radiata (Vr). 1000 times of bootstrap (value in percentage) is marked at each node and the accession number is presented in the parentheses of each sequence; the red branches highlight LOW and its homologs within legume species; the red triangle marks the LOW and blue circles indicate two paralogues from Arabidopsis. DOF5.6 was chosen as an outgroup