Fig. 9
Transient expression of PpCRN7 and PpCRN20 on N. benthamiana leaves followed by P. parasitica infection. a N. benthamiana leaves transiently transformed with the effectors PpCRN7 and PpCRN20 and inoculated with 106 zoospores of P. parasitica. Foliar tissues expressing PpCRN7 and PpCRN20 and inoculated with P. parasitica developed symptoms of severe wilting and necrosis at 72 and 144 h post inoculation (hpi). Controls and agroinfiltrated leaves with PpCRN genes without inoculation with P. parasitica zoospores, and leaves infected with P. parasitica zoospores did not develop visible symptoms. At 144 hpi all infected plants developed symptoms, however, these symptoms were stronger in plants that transiently expressed the PpCRNs. b Differences in DNA detection of leaves infected with P. parasitica (control) and P. parasitica + PpCRN7, P. parasitica + PpCRN20 at 72 hpi are highly statistically significant. c The amount of genomic DNA of P. parasitica is statistically different for control plants, with values of: p-value < 0.000, indicated by the asterisk (Kruskal-Wallis test)