Fig. 6

Analysis of DNA methylation in the VvHB58 gene using bisulfite sequencing. a Structure of VvHB58 gene. Three representative regions (M1, M2 and M3) were selected to detect DNA methylation level in ‘Red Globe’ (R.G). b Schematic diagram of bisulfite sequencing results. Red, blue, and green represent three types of cytosine methylation: symmetric CG, symmetric CHG, and asymmetric CHH (H = A, C, or T) respectively. Representative sequencing results of 5 or 10 single colonies were shown