Fig. 2

MtNOOT1 spatial expression pattern in medicago wild-type primary root. a, b Longitudinal section of root tip. MtNOOT1 expression pattern in root tip determined by in situ hybridization. MtNOOT1 is mainly expressed in the TZ and distal part of the EDZ. b A close-up of (a), visualizing abundance of MtNOOT1 mRNA (red dots) in epidermal and cortical cells in the TZ. Arrowhead indicates the boundary between the RAM and the EDZ of the second layer of cortical cells. c The expression pattern of MtNOOT1 in different zones of root determined by qRT-PCR. Left panel: the primary root of a wild-type medicago seedling at 6 DAG is divided into four zones of 5 mm each. Right panel: MtNOOT1 expression as determined by qRT-PCR reveals increased expression in older regions of the root. The data represent means + SEM of three independent experiments. d Cross section of zone 4. MtNOOT1 expression pattern in zone 4 determined by in situ hybridization. MtNOOT1 is mainly expressed in the procambium cells of the root vascular bundle. Black dotted line circles the pericycle of vascular bundle. Triangles indicate the MtNOOT1 mRNA signals. Scale bar: 100 μm (a), 50 μm (b, d)