Fig. 4

CGF1 and CGF2 are essential for viability. a-b Schematic illustration of the CGF1a or CGF2 genomic locus b and the generation of CRISPR/Cas9 mutants. Arrowheads point at the genomic locus where Cas9 is targeted. c-e Plant height at 7 WAG c, rosette diameters at 4 WAG d, or fresh weight at 4 WAG e of wild type, cgf1–1, cgf1–2, cgf2, cfg1–1/+;cgf2, cgf1–1;cgf2/+, cgf1–2;cgf2, or CGF2g;cgf1–1;cgf2. Results are means ± SD (n = 15). Different letters indicate significant different groups (OneWay ANOVA, Tukey’s multiple comparisons test, P < 0.05). (f) Representative vegetative (top panel) or reproductive growth (bottom panel) of designated genotypes