One-step generation of composite soybean plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation

Table 1 Comparison of transformation efficiency between one-step and two-step ARM transformation methods in Williams 82

Transformation method	dpi	Numbers of plants infected	Numbers of the explants with at least one hairy root	Numbers of hairy roots (per seedling)	Number of GUS-positive roots (per seedling)	Transformation efficiency	Transformation frequency
one-step	16	30	30	5.80 ± 1.40^A	3.10 ± 0.96^A	100%	53.45%
(Cutting)	20	30	30	12.4 ± 3.8 ^A	6.5 ± 2.7 ^A	100%	52.4%
two-step	16	30	14	1.43 ± 1.72^B	0.77 ± 0.97^B	40%	53.85%
(Stabbing)	20	30	24	5.6 ± 1.8^B	1.8 ± 1.2^B	66.7%	32.14%

Transformation root efficiency and frequency calculated at 16 dpi and 20 dpi. Letter A and B represented by different letters are very significantly different at the p = 0.01 value given. Values are mean ± SD for three independent replicates (n = 30 for each replicate)

ISSN: 1471-2229