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Fig. 5 | BMC Plant Biology

Fig. 5

From: Comparative analyses of DNA repeats and identification of a novel Fesreba centromeric element in fescues and ryegrasses

Fig. 5

Graphical layouts of selected DNA repeats and their validation by Southern blotting. Graphical layouts were obtained after clustering analyses done by RepeatExplorer. a Cluster CL1. b Cluster CL38 containing the Ty3/gypsy Athila element. c Cluster CL20 containing Ty3/gypsy Ogre/Tat elements. Sequencing reads from Festuca species are in pink, whereas sequencing reads from Lolium species are in yellow. d–f Validation of clustering results by Southern hybridization with sequences derived from clusters CL1, CL38, and CL20. Lanes contained genomic DNA digested by HaeIII restriction endonuclease. Lane 1: F. pratensis cv. Fure (2n = 2x = 14); lane 2: F. pratensis cv. Westa (2n = 4x = 28); lane 3: F. arundinacea subsp. arundinacea (2n = 6x = 42); lane 4: F. gigantea (2n = 6x = 42); lane 5: F. glaucescens (2n = 4x = 28); lane 6: F. mairei (2n = 4x = 28); lane 7: L. multiflorum cv. Mitos (2n = 4x = 28); lane 8: L. multiflorum cv. Kuri1 (2n = 2x = 14); lane 9: L. perenne cv. Neptun (2n = 4x = 28); lane 10: L. perenne GR 3320 (2n = 2x = 14)

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BMC Plant Biology

ISSN: 1471-2229

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