Fig. 2

Subcellular localization of the GhCIPK6a:GFP fusion protein in onion epidermal cells. a. Fluorescence microscopy images of cells expressing GFP protein. b. Fluorescence microscopy images of cells expressing the GhCIPK6a:GFP fusion. c. Fluorescence microscopy images of plasmolyzed cells in 30% sucrose solution. The cell membrane is marked with a red arrow, and the vacuole membrane is marked with a yellow arrow. Confocal images of epidermal cells were captured 22–24 h after transformation. Bar = 50 μm