Fig. 8

The Arabidopsis Hsp gene expression of WT and TaHsfA2–10-overexpressed line under normal conditions (a) and HS of BT and AT (b-f). Five-day-old T3 generation seedlings of the TaHsfA2–10 transgenic line 11_26 and WT on agar plates were subjected to HS, and then the rosette leaves were sampled at different time interval for qRT-PCR analysis. Meanwhile, the rosette leaves of the TaHsfA2–10 transgenic line 11_26 and WT before two kinds of heat treatments were sampled, respectively. For Hsp genes expression of transgenic line under normal conditions, the value of WT was normalized as 1 (a). For the gene expressions of heat treatments (b-f), the value of 0 h was normalized as 1. Each bar value represents mean ± SD of triplicate experiments, three technical replicates were performed in each experiment, and raw data refer to Additional file 2. Double asterisks indicate the significant differences between WT and overexpressing lines at P < 0.01 level (t-test)