Fig. 6
From: OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice
OsCAF2 interacts with itself through the N-terminal region. a Y2H interaction of OsCAF2 with itself. The full-length CDS of OsCAF2 was cloned into BD and AD vectors, to produce OsCAF2-BD and OsCAF2-AD vectors, respectively. The OsCAF2-BD and OsCAF2-AD were co-transformed into the AH109 yeast strain. The OsCAF2-BD and AD plasmid, and the OsCAF2-AD and BD plasmid were co-transformed into the AH109 yeast strain as control. b Y2H interaction of OsCAF2 with itself. The truncated sections of OsCAF2 were cloned into BD and AD vectors to produce the OsCAF2-N-BD, OsCAF2-M-BD, OsCAF2-C-BD, OsCAF2-N-AD, OsCAF2-M-AD and OsCAF2-C-AD vectors. A pair of AD and BD was co-transformed into the AH109 yeast strain. The yeast cells were cultured on SD-T/L and SD−/T/L/H/A media with different dilution series (1, 10− 1, and 10− 2)