Fig. 6

Overview of transcriptome sequencing data of rapeseed plants under the control and salt conditions. a Correlation analysis between the RT-qPCR assays and RNA-seq results. b, c Principal component analysis (B) and Venn diagram analysis (c) of the differentially expressed genes (DEGs) in the shoots (S) and roots (R) between the control and salt (treatment) conditions. The DEG numbers are listed in the brackets. d-e Volcano diagrams showing the DEGs between the control and salt (treatment) conditions in the shoots (d) and roots (e). Up, upregulation; down, downregulation. f-g GO (f) and KEGG pathway (g) enrichment analysis of global DEGs in the shoots and roots between the control and salt (treatment) conditions. For f, BP, biological process; CC, cellular component; MF, molecular function. Over-presentation of the GO items are delineated by the WordArt program. The bigger the font size, the more the corresponding GO categories. For g, the circle size indicates the number of DEGs, and the rich factor indicates the degree of enrichment of KEGG pathways involving the DEGs. For the transcriptome sequencing, uniform rapeseed plants after 7-day seed germination were cultivated under NaCl-free (control) for 10 days, and then they were changed to the solution containing 200 mM NaCl for 12 h