Table 1 Primers used for PCR and qRT-PCR
From: Investigation of the role of AcTPR2 in kiwifruit and its response to Botrytis cinerea infection
Purpose | Name | Sequence (5′-3′) | |
|---|---|---|---|
AcTPR2-pTRV2 construction | AcTPR2 | F | (XbaI)GCTCTAGAATTACTAGCACCAGCGGCAC |
R | (BamHI)CGGGATCCAGGTCGAGGACAACCGTTAC | ||
PCR | ITS | F | TCCGTAGGTGAACCTGCGC |
R | TCCTCCGCTTATTGATATGC | ||
qRT-PCR | ITS | F | CTGTTCGAGCGTCATTTCAA |
R | CCTACCTGATCCGAGGTCAA | ||
qRT-PCR | Actin | F | GCAGTGTTTCCCAGTATTGT |
R | TCCATGTCATCCCAGTTGC | ||
qRT-PCR | AcTPR2 | F | GGGCTGCGTATACTGAGGAT |
R | TTGCTTGCTGTCAAAGGGTC | ||
qRT-PCR | AcNIT1 | F | TTTGTTTTGTCAGCCAACCA |
R | ATGCCCAACCACATCAAAAT | ||
qRT-PCR | AcARF1 | F | TTGTGTGCCATTAGGCATGT |
R | TGGTTTCCACTTGGTTAGGC | ||
qRT-PCR | AcARF2 | F | AGGGTCTTGCAAAGTCGAGA |
R | GATCGAGTCCCAAAACCTGA | ||