Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

Table 3 Segregation of genotypes at DNA markers for QTLs and associated rate of preharvest sprouting

Marker name	plant no.	plant number of each genotype			Ratio of pre-harvest sprouting (%)			One-way ANOVA
Population	plant no.	AA	AB	BB	AA	AB	BB	F-value	P
*qPHS1_KY29_PHS_17515*
Cross A_1	130	29	69	32	23.3 ± 23.3	10.9 ± 18.1	4.5 ± 7.1	9.14	0.0020 *
Cross A_2	106	21	62	23	11.2 ± 16.4	9.1 ± 12.3	6.2 ± 6.5	0.93	0.4137
*qPHS6_KY29_WG_26859*
Cross A_1	130	35	59	36	25.5 ± 26.8	8.4 ± 13.4	8.7 ± 12.6	7.13	0.0057 *
Cross A_2	106	20	61	25	9.5 ± 9.6	10.1 ± 13.9	5.4 ± 9.3	1.35	0.2865
*qPHS1_KY28_Sh_3180*
Cross B_1	100	40	42	18	59.4 ± 30.9	69.0 ± 24.5	69.4 ± 25.5	1.51	0.2483
Cross B_2	106	34	49	23	60.6 ± 28.5	58.8 ± 25.4	55.0 ± 23.2	0.32	0.7309
*qPHS3_KY28_WG_1572*
Cross B_1	100	27	49	24	77.7 ± 24.7	67.0 ± 25.8	47.7 ± 25.8	9.03	0.0021 *
Cross B_2	106	34	52	20	70.1 ± 21.3	57.5 ± 25.2	41.8 ± 23.0	8.75	0.0024 *
*qPHS4_KY28_PHS_921*
Cross B_1	100	26	50	24	78.4 ± 21.6	67.4 ± 27.1	46.7 ± 25.0	10.21	0.0012 *
Cross B_2	106	22	59	25	57.0 ± 26.3	61.5 ± 25.6	53.0 ± 26.1	0.99	0.3906
*qPHS8_KY28_WG_21775*
Cross B_1	100	18	49	33	50.5 ± 30.7	60.7 ± 27.6	80.1 ± 17.8	9.48	0.0017 *
Cross B_2	106	23	57	26	43.5 ± 19.2	57.2 ± 26.8	75.0 ± 19.5	11.01	0.0009 **

ISSN: 1471-2229