Fig. 8

Effects of silencing NbABA1 or NbABA2 on CWMV CP accumulation. a Phenotypes of NbABA1-silenced and NbABA2-silenced plants. Photograph taken 7 d post agro-infiltration. b RT-qPCR results of relative expression levels of NbABA1 and NbABA2. c RT-qPCR results of relative expression levels of NbRAB18 and NbABI5 in NbABA1-silenced plants. d RT-qPCR results of relative expression levels of NbRAB18 and NbABI5 in NbABA2-silenced plants. Means ± SE were calculated from three biological replicates relative to TRV:00 plants, and each replicate comprised three technical replicates. **, P < 0.01 (Student’s t-test). e RT-qPCR results of mRNA expression of CWMV CP in NbABA1-slienced plants. Means ± SE were calculated from three biological replicates relative to CWMV-infected TRV:00 plants, and each replicate comprised three technical replicates. **, P < 0.01 (Student’s t-test). f Western blot showing protein expression of CWMV CP in NbABA1-slienced plants. Coomassie brilliant blue-stained loadings are shown in the lower section of the figure. g RT-qPCR showing mRNA expression of CWMV CP in NbABA2-silenced plants. Means ± SE were calculated from three biological replicates relative to CWMV-infected TRV:00 plants, and each replicate comprised three technical replicates. **, P < 0.01 (Student’s t-test). h Western blot assay showing protein expression of CWMV CP in NbABA2-slienced plants. Coomassie brilliant blue-stained loadings are shown in the lower section of the figure