Fig. 3

The phenotype and genetic differences of Huagu11 and Yugu1 in the resistance to the herbicide imazethapyr. a The phenotypes of Huagu11 and Yugu1 after treatment with the herbicide imazethapyr. The Yugu1 leaves wilted, withered, and died 7 days later after the treatment of 0.25% Imazethapyr, whereas Huagu11 grew normally. b The protein alignment of AHAS from Huagu11 and Yugu1. The red letters indicate different amino acids. c Comparative sequence alignment of AHAS showing phylogenetic relationships among members of the grass lineage. The rectangular box indicates the position of the amino acid substitution providing resistance against imidazolinone herbicides. The red * after the cultivars indicates that the cultivar has resistance against imidazolinone herbicides. The * under the sequences indicates the identical position of amino acids in all cultivars