Fig. 7

Microautophagy-related genes are transcriptionally induced in stressed fc2 seedlings The induction of microautophagy was assessed in fc2-1 seedlings. A Shown is a representative TEM image of the invagination of the vacuolar membrane associated next to a degrading chloroplast in the fc2-1 atg5 mutant. c; cytoplasm, cv; central vacuole, mi; microautophagy structure, p; plastid, t; tonoplast. B A Venn diagram showing overlap of putative microautophagy-related genes and autophagy-related genes used in this study (Tables S1 and S2). C A heatmap of microautophagy-related gene expression (relative to wt) in etiolated seedlings at time points prior to (0 min) and during de-etiolation (30 min and 120 min). Microarray data was previously generated from etiolated seedlings grown in the dark for four days at time points before and after light exposure [15]. The list of genes considered is presented in Table S2. D RT-qPCR analysis of select microautophagy-related transcripts from four-day-old seedlings grown under 6 h light/18 h dark light cycling conditions. Shown are mean values ± SEM (n = 6 biological replicates). Statistical analyses were performed by student’s t-tests. *, **, *** indicate a p-value of ≤ 0.05, ≤ 0.01, and ≤ 0.001, respectively. In all bar graphs, closed circles represent individual data points