Fig. 3

Consensus phylogram of aminocyclopropane-1-carboxylic acid synthase (ACS) proteins from Arabidopsis, tomato and kiwifruit. Predicted ACS proteins from Arabidopsis (At), tomato (Sl), and kiwifruit (Ac/blue dot) were aligned using the Geneious MUSCLE alignment tool and a consensus UPGMA bootstrap phylogram was generated using 1000 replicates (Jukes-Cantor distance matrix). Only branches with over 50% support threshold are displayed. AT = aminotransferase. Type I, II and III ACS proteins were assigned based on presence of conserved C-terminal sequences (see Supplemental Fig. S1 for alignment). Type I (red) = RLSF/SLSF only; type II (green) = WVF, RLSF and RDE rich domains (TOE/target of ETO1 domain); type III (blue) = absence of type I/II domains (based on Yoshida et al. 2006) [57]. (III) = Both SlACS4 and 13 cluster with type I, but show absence of typical type I residues in the C-terminus (see Supplemental Fig. S1 for alignment). S1, S2 = involved in system-1, − 2 ethylene production. T = involved in transition between system-1 and -2