Fig. 6

(A) Ethylene production in wounded kiwifruit leaves. Expanding A. chinensis ‘Hort16A’ leaves were wounded with a 96-well pin blotter and ethylene production was measured continuously over the time course shown (0, 1.5, 3, 6 h after wounding). Data are mean ± SD, n = 3 biological replicates. (B) qRT-PCR of ethylene biosynthetic (ACO and ACS) genes and NAC TFs in wounded leaves and control leaves (0, 3, 6 h after wounding). Data are mean ratio calibrated ± SD, n = three biological replicates and expressed as a ratio compared to the PP2A reference gene. **: p < 0.01 significantly different compared to unwounded leaves, based on Student’s t-test (A) or mixed model statistics (B) (R packages nlme and emmeans, as described in the Materials and Methods)