Genome-wide identification, gene cloning, subcellular location and expression analysis of SPL gene family in P. granatum L

Table 3 Primers used in this study

Primer	Primer sequence(5′-3′)	Annotation
PgSPL5	F: ATGTTGGACTATGAATGGGC R: TCACCCGCTAGAGAAAAAGAG	Gene clone
PgSPL13	F: ATGGGATCAAGAAGAAACCTCGAAG R: TCAGTTTGAGCCTTCTCCAGGAAAA	Gene clone
GFP-PgSPL5	F:gagaacacgggggactctagaATGTTGGACTATGAATGGGC R:gcccttgctcaccatggatccTCACCCGCTAGAGAAAAAGAG	subcellular localization
GFP-PgSPL13	F:gagaacacgggggactctagaATGGGATCAAGAAGAAACCTCGAAG R:gcccttgctcaccatggatccTCAGTTTGAGCCTTCTCCAGGAAAA	subcellular localization
qRT-PgSPL2	F:AAGAGAGACTTCACCACCGAG R: GTGAAACCTGCTGCATTGCT	Gene expression
qRT-PgSPL3	F: AACAGCCGAGACTCATCCC R: GAGGTCCTTGTTACACCCGTA	Gene expression
qRT-PgSPL5	F: GAAATCTCAGCAACAGCCGAAT R: CTTTGCCTGAAACAAGAGTCG	Gene expression
qRT-PgSPL6	F: AATGCAGCAGGTTTCATTCCC R: TCGTTTTCCGACTTGACAGC	Gene expression
qRT-PgSPL11	F: TTCCCAGCCATTCCATCCTCG R: GGCTTCGCTATACCAAGTCCT	Gene expression
qRT-PgSPL12	F: AAGTCCCCTAAAGTCATCGTT R: TGAAGTCCATCACAAAGCCT	Gene expression
qRT-PgSPL13	F: CGACAACTGCACGACCGAT R: CGGCAGCTTCTTTTCGCTTC	Gene expression
qRT-PgSPL14	F: TCGACGATACCAAGAGAAGCTG R: AATTCTCCCCCGATCATCGAC	Gene expression
PgActin	F: AGTCCTCTTCCAGCCATCTC R: CACTGAGCACAATGTTTCCA	Gene expression

ISSN: 1471-2229