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Fig. 2 | BMC Plant Biology

Fig. 2

From: Identification of miRNAs-mediated seed and stone-hardening regulatory networks and their signal pathway of GA-induced seedless berries in grapevine (V. vinifera L.)

Fig. 2

Screening of novel VvmiRNAs at grape stone-hardening stage. a First base preference. b Distribution in grape chromosomes of VvmiRNAs. c Validation of VvmiRNAs from high throughput sequencing data by qRT-PCR at different grape berry developmental time points [YB (5DAF), SB (45DAF), MB (90DAF)]. Each bar indicated the mean ± SE of triplicate assays. H: high throughput sequencing; Q: qRT-PCR; each assay was conducted by using three repeats. There is no significant difference between high throughput sequencing and qRT-PCR results in P < 0.05 by Student’s t-test. d Comparison of specific and significant VvmiRNAs in grape sRNA library. VvmiRNAs with specific expressions at the seed coat-hardening stage, Spec-VvmiRNAs: VvmiRNAs with the absolute log2Fold (YB/SB) or log2Fold (MB/SB) > 1, Significant difference VvmiRNAs

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BMC Plant Biology

ISSN: 1471-2229

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