Table 2 Screening results of each barcode primer
From: Developing an efficient DNA barcoding system to differentiate between Lilium species
Gene Name | Length (bp) | Primer Name | Forward primer | Reverse primer |
|---|---|---|---|---|
trnF-ndhJ | 855bp | LHV3 | TGGATATAGACCTCCATTTTTGAG | GATAATGACACGACTCCAGAA |
trnS-trnG | 665bp | S1 /LHV1 | CTCTCCCAACTCAAAATTG | CAGAATTATGAAAATTATAGCGT |
trnP-psaJ-rpI33 | 633bp | S5/LHV5 | ATCCTTGTCTTGTTTTCCAC | TTCTAACTMTCAATTATTCCTA |
psbB-psbH | 1124bp | LHV6 | GGGTTGGTTCACTTTTGGGC | TCCACGGTCGAACTACCAGA |
ycf1b | 750bp | LHV10 | ACCACCCGTTTGGCTTTTCT | CCATGCCCATTTCCGGTTTG |
matK | 800bp | matK | CGATCTATTCATTCAATATTTC | TCTAGCACACGAAAGTCGAAGT |
rbcL | 600bp | rbcL | ATGTCACCACAAACAGAGAC | TCACATGTACCCGCAGTAGC |
psbA-trnH | 400bp | psbA-trnH | ACTGCCTTGATCCACTTGGC | CGAAGCTCCATCTACAAATGG |
trnL-trnF | 700bp | trnL-trnF | CGAAATCGGTAGACGCTACG | ATTTGAACTGGTGACACGAG |
ITS | 750bp | ITS | GGAAGKARAAGTCGTAACAAGG | RGTTTCTTTTCCTCCGCTTA |