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Fig. 4 | BMC Plant Biology

Fig. 4

From: Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu)

Fig. 4

PEG-mediated transformation of areca palm protoplast. a PEG-mediated transformation of areca palm protoplast at 48 h post-transfection (hpt). 3 × 104 areca protoplasts were transformed with 3 μg of a green fluorescent protein (GFP)-expressing plasmid (pGreen0029-GFP, 6.0 kb) and observed under a fluorescent microscope. Scale bar = 100 μm; b Close view of pGreen0029-GFP expression in areca protoplast at 48 hpt. Scale bar = 20 μm

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