Fig. 5
Effect of protoplast density on PEG-mediated transformation of areca palm protoplasts. a GFP imaging of areca palm protoplasts transformed with pGreen0029-GFP at various cell densities of 25, 50, 100, 150 and 200 × 104 cells/ml, respectively at 48 hpt. Scale bar = 100 μm. b Effects of protoplast density on transfection efficiency. Areca protoplasts at various cell densities (30 μl each) were transfected with 3 μg of pGreen0029-GFP plasmid DNA, respectively. Transfection efficiency was calculated as the percentage of green fluorescent protoplasts divided by the total number of protoplasts. Error bars represent the SD of three replicates. Different lowercase letters represent significant statistical difference