Fig. 4

NIA1 and NOA1-dependent NO were essential for CAG-induced stomatal movement in A. thaliana. a After exogenous c-PTIO, L-NAME and Na₂WO₄ were applied, the stomatal apertures of A. thaliana leaves under the CAG treatment in WT. b and c Under different treatments, 10 μM fluorescent dye DAF-2DA was added in the dark for 15 min to measure the fluorescence intensity of NO in guard cells, and the stomatal images were taken. Bar = 2 μm. d Stomatal apertures of A. thaliana under the CAG treatment in WT, nia1, nia2, and noa1. e and f Under CAG treatment, the fluorescence intensity of NO in WT, nia1, nia2, noa1, and the stomatal images were taken. Bar = 2 μm. Uppercase letters indicate differences between groups, lowercase letters indicate differences within groups. Means with different letters are significantly different at P < 0.05