Fig. 8

Characteristics and function of LiTTG1-1. (A) A neighbor-join tree of the TTG1 proteins involved in flavonoid biosynthesis. AtTTG1, Arabidopsis thaliana, accession number AJ133743; GhTTG, Gossypium hirsutum (GhTTG1, accession number AF336281; GHTTG2, accession number AF530912; GhTTG3, accession number AF530911; GHTTG4, accession number AF530910); MdTTG1, Malus domestica, accession number AF220203; LiTTG1, Lagerstroemia indica. PhAN11, Petunia hybrida, accession number U94748; PgTTG1, Punica granatum (pomegranate), accession number HQ199314; Vicia faba, VfTTG1, accession number MN119531; Zea mays, PAC1, accession number AY115485; Zea mays, MP1, accession number AY339884. (B) Transcriptional level of LiTTG1s among the three colored petals. FPKM, fragments per kilobase million. (C) The phenotype of tobacco leaves. The injection combinations were indicated above the leaves and circles indicated the injection zone. (D) Total anthocyanin. (E–G) Relative expression levels of the NbDFR(E), NbANS(F), and NbUFGT(G). Internal control, Actin gene; Sample control, empty pWM101 vector. Mean ± SD. All data were collected from at least three individual plants and three technical repeats. One-way ANOVA was used to analyze differences among samples