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Table 2 Effect of Phytophthora sojae infection on stem lipidome of susceptible and tolerant soybean cultivars

From: Lipid mediated plant immunity in susceptible and tolerant soybean cultivars in response to Phytophthora sojae colonization and infection

Lipid classes

Lipid sub-classes

Relative abundance (nmole%)

OSC

OSI

CSC

CSI

Glycerophospholipids

PC*

22.05 ± 2.50b

17.52 ± 0.92c

20.98 ± 0.26b

23.74 ± 0.39a

PE*

33.89 ± 2.13a

27.45 ± 1.48b

21.18 ± 0.18d

22.25 ± 0.61c

PA*

2.72 ± 1.27d

7.60 ± 1.52c

8.39 ± 0.20b

13.37 ± 1.88a

PG*

8.77 ± 1.16c

7.80 ± 0.41d

12.30 ± 0.69a

10.72 ± 1.56b

PI*

5.16 ± 0.59c

4.54 ± 0.50d

6.67 ± 0.41b

7.26 ± 0.64a

PS*

2.24 ± 0.66b

1.25 ± 0.63c

5.33 ± 0.76a

2.91 ± 0.40b

LPCns

0.42 ± 0.04

0.32 ± 0.03

0.38 ± 0.04

0.36 ± 0.05

LPEns

0.09 ± 0.00

0.08 ± 0.02

0.09 ± 0.00

0.06 ± 0.01

Glycerolipids

TG*

10.11 ± 1.25d

16.55 ± 0.02b

17.89 ± 1.50a

11.66 ± 2.00c

DG*

7.78 ± 1.15b

8.35 ± 0.72a

2.62 ± 0.10d

4.45 ± 0.12c

Phytosterols

AcHexSiEns

0.55 ± 0.06

0.72 ± 0.14

0.11 ± 0.02

0.60 ± 0.01

SiE*

1.14 ± 0.25a

0.46 ± 0.20b

0.68 ± 0.11a

0.41 ± 0.05b

AcHexStEns

0.75 ± 0.37

0.69 ± 0.21

0.04 ± 0.00

0.05 ± 0.01

AcHexCmEns

0.14 ± 0.05

0.20 ± 0.03

0.01 ± 0.00

ND

CmEns

0.44 ± 0.12

0.15 ± 0.05

0.01 ± 0.00

0.01 ± 0.01

StE*

0.34 ± 0.16b

1.98 ± 0.02a

0.33 ± 0.05b

0.40 ± 0.15b

Sphingolipids

HexCer*

0.89 ± 0.17b

1.96 ± 0.02a

1.00 ± 0.08b

1.67 ± 0.03a

Cerns

0.27 ± 0.06

0.31 ± 0.02

0.71 ± 0.04

0.21 ± 0.03

SMns

ND

ND

0.03 ± 0.01

0.01 ± 0.00

Glyceroglycolipid

MGDG*

2.25 ± 0.08a

2.09 ± 0.06a

1.25 ± 0.18b

0.47 ± 0.07c

Total

100.00

100.00

100.00

100.00

Glycerophospholipids*

75.34 ± 1.20b

66.56 ± 1.32c

75.32 ± 1.22b

80.67 ± 2.15a

Glycerolipids*

17.89 ± 0.25c

24.90 ± 1.51a

20.51 ± 1.60b

16.11 ± 1.13d

Phytosterols*

3.36 ± 0.12b

4.18 ± 0.66a

1.18 ± 0.19c

0.86 ± 0.06d

Sphingolipids*

1.16 ± 0.03c

2.27 ± 0.05a

1.74 ± 0.12b

1.89 ± 0.06b

Glyceroglycolipid*

2.25 ± 0.08a

2.09 ± 0.06a

1.25 ± 0.18b

0.47 ± 0.07c

  1. Values in the table (nanomole% by weight composition) denote means ± standard errors for four biological replicates. Means in the same row with different superscripts are indicated as significantly different (*significant at alpha = 0.05) or not significantly different (ns) between the treatments, which consisted of susceptible control (OSC) and inoculated (OSI) stem tissue; and tolerant control (CSC) and inoculated (CSI) stem tissue from 10-day old seedlings, at a significance level of α < 0.05. The lipids detected were: PA Phosphatidic acid, PE Phosphatidyl- ethanolamine, PC Choline, PG Glycerol, PS Serine, PI inositol, TG triacylglycerol, DG Diacylglycerol, LPC Lysophosphatidylcholine, LPE Lysophosphatidylethanolamine, SM Sphingomyelin, MGDG Monogalactosyldiacylglycerol, SiE Beta sitosterol, StE Stigmasterol ester, HexCer Hexosyl ceramide, Cer Ceramide, CmE Campesterol ester, AcHexStE Acylated hexosyl stigmasterol ester, AcHexSiE Acylated hexosyl  betasitosterol ester, and AcHexCmE Acylated hexosyl campesterol ester. Lipids that were not detected (ND) under the treatment conditions are indicated