Table 5 Amount of each solution used for reaction
Reagent (enzyme) | Dosage (mL) | Final concentration |
|---|---|---|
0.05Â mol/L potassium phosphate buffer | 1. 5 | Â |
130Â mmol/L methionine | 0.3 | 13Â mmol/L |
750 μmol /L NBT | 0.3 | 75 μmol/L |
100 μmol /L EDTA-Na2 | 0.3 | 10 μmol/L |
20 μmol /L riboflavin | 0. 32 | 0 μmol/L |
the enzymatic extract | 0.05 | Two control tubes with buffer solution |
Distilled H2O | 0.25 | Â |
Total volume | 3.0 | Â |