Fig. 3From: Application of bulk segregant RNA-Seq (BSR-Seq) and allele-specific primers to study soybean powdery mildew resistanceDifferential expression analysis. (a) Venn diagram showing the consensus 1,687 DEGs identified by comparing the resistant and susceptible parents as well as bulks using both HISAT2-DESeq2 and Kallisto-Sleuth pipeline. (b) Heatmap of the consensus 1,687 DEGs represented by DESeq2 quantification. (c) Mean separation test for PM incidence in the F2 generation according to the AS-Chr16 genotype, and (d) the Satt431 genotype. The Kruskal-Wallis test and the Dunn’s test was applied to determine significant difference at α = 0.01Back to article page