Fig. 6

Transient expression of OsCYCDs triggers tobacco pavement cells to divide. (A) Differentiated leaf pavement cells are outlined by microtubules at the cell cortex without ectopic expression of any OsCYCDs. (B) Upon OsCYCD2;2 expression, leaf pavement cells enter mitotic cell division, obvious mitotic signs such as spindles and phragmoplasts were observed in green. (C) Seventy-two hours after infiltration of agrobacteria with OsCYCD2;2, most if not all pavement cells have already completed cell division as marked by cell plates (red arrows). An induced pavement cell forms a preprophase microtubule band (D), and cells in early-prophase (E), propahse (F), metaphase (G), anaphase (H), early-cytokinesis (I), late-cytokinesis (J), and completed division (K). Microtubules are marked by GFP-TUA6 (green) and nuclei highlighted by AtHistone H1.2-TagRFP (red). Bars: (A–C) 100 μm; (D–K) 10 μm