Fig.1

The K0-OsNAC02/OsNAC06 mutant (T2) construction and botanic characters detection. Two mutants named N2, N3 are planted in field (Fuyang, Hangzhou in 2021), and the homozygous was harvested in the autumn. The control is wild type of Nipponbare. The genetic detection of all homozygous mutants was performed by PCR sequencing. The N2 has two mutant sites in OsNAC02 CDS regions, N3 has one mutant site in OsNAC02 and OsNAC06 CDS regions respectively (Fig. S1). a N2 mutant construction. The K0-Osnac02 mutant created by Osnac02 -tag2 has 7 bp-deletion in N2 mut and K0-Osnac02 mutant has 1bp-deletion in N3 mut, respectively. b N3 mutant construction. The K0-Osnac06 mutant created by Osnac06 -tag2 has 3 bp-deletion in N3 mut. c The N2/N3 mut plants (95DAG) are selected for botanic characters detection (Fig. S2). n = 17.5 cm. d The premature phenotype in N2/N3 mut (95DAG) and the development period calculation of the mutants (two-way ANOVA for mixed model, *, p < 0.05 and **, p < 0.01, n = 6 mm). The mutant matured earlier than WT as a result. For the detection of premature phenotypes in N2 and N3 mut, the seed shape characters such as length, width, and thickness are collected in Fig e and Fig f. e The shape phenotypes detection of N2/N3 mut seeds (95 DAG), and there are no difference between mut vs WT. f The shape phenotypes of full-mature seeds (T2) in N2/N3 mut (125 DAG) are detected, which were harvested in 2021. The full-filled seeds (125 DAG) of WT is larger than mutants on the whole