Fig. 2

The functional analysis of OsNAC02 and its co-network construction. a The distinct expressions of OsNAC02 and OsNAC06 were verified by qRT-PCR in different tissues. Both of them expressed highly in root, as this organ is most vegetative parts of WT seedling. The ubiquitin (LOC_Os03g13170.1, Os03g0234200) was used as the reference which expressed in the whole plant. b The distinct tissue expressions of OsNAC02 (LOC_Os02g38130, Os02g0594800) on NCBI, and this result is in accordance with Fig a. c Yeast-one-hybrid assays show the OsNAC02 interact with the promoter fragments of OsHSFA2C, OsHSFA2E, OsMYB04. The positive control in blue colonies was constructed according to the reference [24]. There are two cultures in this experiment used as the selection medium: SD and SD /-Ura/-Trp. The X-gal culture is SD /-Ura/-Trp + DMF + X-gal with the content of 10–1 and 10-2 for positive screen. d The qRT-PCR results represent the expressions of OsNAC02 regulated genes in the leaf and root of mutants vs WT, including: OsNAC02, OsNAC06, OsMYB04, OsHSF2B, OsHSFA2C, OsHSFA2E (Table S12). The ubiquitin (LOC_Os03g13170.1) is used as the reference gene (student t-test, *, p < 0.05 and **, p < 0.01), and the motif locations are listed in the results (Fig. S5a)